General Cell Based Assays
Eurofins Discoverx PathHunter Human GPR85 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human GPR85 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure GPR85 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Meso Scale Discovery R-PLEX Human Cathepsin L2 ASY
The R-Plex Human Cathepsin L2 Assay is a multiplex immunoassay kit that enables simultaneous quantitative measurement of human Cathepsin L2 protein in cell and tissue lysates It uses a quantitative sandwich immunoassay technique and color-coded magnetic beads for multiplex analysis The assay has a detection range of 78-50 000 pg/mL and includes a 96-well plate assay buffer wash buffer serum matrix standards detection antibodies streptavidin-PE and magnetic beads It can help researchers study Cathepsin L2 s role in cancer bone remodeling and protein processing The assay requires a Luminex-based reader and xPONENT software for analysis
ABclonal Technology ADAM17 Rabbit pAb
This gene encodes a member of the ADAM (a disintegrin and metalloprotease) family, which includes membrane-anchored proteins related to snake venom disintegrins. These proteins are involved in various biological processes such as fertilization, muscle development, and neurogenesis. The preproprotein is processed to produce the mature protease, which sheds the ectodomain of tumor necrosis factor-alpha (TNF-alpha), releasing soluble TNF-alpha. This protease also processes cell adhesion proteins, cytokine and growth factor receptors, and EGF receptor ligands, and activates the Notch signaling pathway. Elevated expression of this gene has been linked to psoriasis, rheumatoid arthritis, multiple sclerosis, and Crohn’s disease, suggesting a role in autoimmune diseases. Additionally, the protease may play a role in viral infection by cleaving ACE2, the receptor for SARS-CoV and SARS-CoV-2.
Eurofins Discoverx PathHunter eXpress GLP2R CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0572E2CP2M)
The PathHunter GLP2R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GLP-2) based activation of GLP2R GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GLP2R and EA-tagged β-Arrestin-2. Activation of GLP2R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human Phospho-AKT3 (Ser473) Detection Kit, 50,000 Assay Points
HTRF Human Phospho-AKT3 (Ser473) Detection Kit, 50,000 Assay Points
Eurofins Discoverx PathHunter eXpress EGFR/EGFR Dimerization Assay, 2-Plate Kit. (93-0989E3CP5M)
The PathHunter EGFR/EGFR Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. EGF) induced dimerization of EGFR/EGFR receptors. This product uses EFC technology. The cell line included overexpresses EGFR target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/EGFR, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc Mouse cAMP response element bi
Mouse cAMP response element binding protein (CREB) ELISA Kit from CUSABIO Detection Range 3 12 pg/mL-200 pg/mL Sensitivity 0 78 pg/mL Sample serum plasma cell culture supernates Method Sandwich
Eurofins Discoverx PathHunter Human P2RY1 β-Arrestin Cell-Based Assay Kit (U2OS)
The PathHunter Human P2RY1 β-Arrestin Stable Cell Line Assay (U2OS) contains a stable clonal cell line used to measure P2RY1 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human CNR2 (CB2) β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human CNR2 (CB2) β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure CNR2 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Cayman Chemical 3-AcetylpyrIdIn NAD 250mg
A NAD analog; used to study the mechanisms of oxidative phosphorylation
Eurofins Discoverx cAMP Hunter eXpress HRH3 CHO-K1 GPCR Assay, 2-Plate Kit. (95-0103E2CP2M)
The cAMP Hunter HRH3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. histamine) based activation of HRH3 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type HRH3, a naturally Gi/Go coupled GPCR. Upon activation of HRH3, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay, 2-plate Kit. (96-0038E1CP7M)
The InCELL Hunter eXpress HEK 293 BRD3(1,2) Bromodomain Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of BRD3(1,2) via detection of BRD3(1,2) levels. This product uses EFC technology. The cell line included overexpresses BRD3(1,2) target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Abcam TET Hydroxylase Activity Quantification Kit (Fluorometric)
TET Hydroxylase Activity Quantification Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Echelon Biosciences Research Labs PI(3,4,5)P3 DiC16 1 mg
PI(3,4,5)P3 diC16 (Phosphatidylinositol 3,4,5-trisphosphate diC16) is a synthetic, purified dipalmitoyl PI(3,4,5)P3.Phosphoinositides (PIPns) are minor components of cellular membranes but are integral signaling molecules for cellular communication. Phosphatidylinositol 3,4,5-trisphosphate (PIP3), formed from PI(4,5)P2 though phosphorylation by PI 3-kinase, activates numerous signaling pathways resulting in cell proliferation, growth, survival, glucose transport and protein synthesis. High PIP3 levels from disregulation of PI3-K have been demonstrated in cancer and inflammatory diseases. PIP3 is hydrolyzed by the phosphatases PTEN to PI(4,5)P2 and SHIP to PI(3,4)P2. This item is non returnable
Eurofins Discoverx PathHunter Human IL-11 (IL11RA/IL6ST) Dimerization Cell-Based Assay Kit (HEK 293)
The PathHunter Human IL-11 (IL11RA/IL6ST) Stable Cell Line Dimerization Assay (HEK 293) measures ligand-induced dimerization of IL11R/IL6ST. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.