General Cell Based Assays
Eurofins Discoverx cAMP Hunter eXpress ADRA2C CHO-K1 GPCR Assay, 2-Plate Kit. (95-0053E2CP2M)
The cAMP Hunter ADRA2C eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. epinephrine and norepinephrine) based activation of ADRA2C GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type ADRA2C, a naturally Gi/Go coupled GPCR. Upon activation of ADRA2C, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Bioassay Systems EnzyLight™ ADP Assay Kit. For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism.
For rapid, quantitative, bioluminescent determination of ADP concentration and evaluation of drug effects on ADP metabolism. Key Features: Safe. Non-radioactive assay. Sensitive and accurate. As low as 0.02 µM ADP can be quantified. Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved. Robust and amenable to HTS: Z factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Method: Luminescence; Samples: Cells etc; Size: 100 tests.
Meso Scale Discovery R-PLEX Human EGFR ASY
The R-PLEX Human EGFR Assay Kit from Meso Scale Diagnostics is a multiplex immunoassay measuring total EGFR levels in human serum plasma and cell culture supernatants This electrochemiluminescence (ECL) assay uses MSD s SULFO-TAG labels and multi-array plate technology for simultaneous detection of multiple protein biomarkers from a single sample It offers high sensitivity broad dynamic range low sample volume and rapid results The kit includes a 96-well plate with capture antibody detection antibody calibrator and assay diluent suitable for biomarker discovery disease research and drug development
Eurofins Discoverx PathHunter eXpress CSF1R/CSF1R Dimerization Assay, 2pl Kit. (93-1061E3CP5M)
The PathHunter CSF1R/CSF1R Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. M-CSF) induced dimerization of CSF1R/CSF1R receptors. This product uses EFC technology. The cell line included overexpresses CSF1R target protein fused to PK alongside CSF1R target protein fused to EA. Upon ligand-induced dimerization of CSF1R/CSF1R, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx cAMP Hunter Teriparatide Bioassay Kit, 10pl. (95-0118Y2-00058)
The cAMP Hunter Teriparatide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Teriparatide based activation of PTHR GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type PTHR, a naturally Gs coupled GPCR. Upon activation of PTHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Abcam Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
Glycerol-3-phosphate (G3P) Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress GPR123 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0417E2ACP1M)
The PathHunter GPR123 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR123 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR123 and EA-tagged β-Arrestin-2. Activation of GPR123 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Molecular Devices FLIPR Calcium 6-QF Assay Kit, Quench-free, 10 plates
The FLIPR Calcium 6-QF Assay Kit (Explorer kit) is a quench-free, mix-and-read fluorescent assay for measuring intracellular calcium flux in high-throughput plate formats. The Calcium 6-QF formulation provides an increased signal window and is suitable for quench-sensitive targets and multiplexed assays.
- Quench-free formulation for quench-sensitive targets and multiplexing.
- Designed for mix-and-read workflows with pre-optimized FLIPR and FlexStation protocols.
- Sufficient reagents for 10 plates in Explorer kit format.
- Each reagent vial for Sample and Explorer kits is sufficient for one 96-, 384-, or 1536-well plate.
- Suitable for endogenous, primary, and stem cell targets with improved signal window and assay robustness.
Eurofins Discoverx cAMP Hunter eXpress GHRHR CHO-K1 GPCR Assay, 10-Plate Kit. (95-0076E2CP2L)
The cAMP Hunter GHRHR eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. GRF) based activation of GHRHR GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GHRHR, a naturally Gs coupled GPCR. Upon activation of GHRHR, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OPRK1 U2OS β-Arrestin GPCR Assay, 2-Plate Kit. (93-0234E3CP5M)
The PathHunter OPRK1 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. dynorphin A) based activation of OPRK1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged OPRK1 and EA-tagged β-Arrestin-2. Activation of OPRK1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress rGPR35 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0887E2RCP2M)
The PathHunter rGPR35 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. zaprinast) based activation of rGPR35 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged rGPR35 and EA-tagged β-Arrestin-2. Activation of rGPR35 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Rat version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
American Research Products Inc ELF5 Transcription Factor Activity Assay
ELF5 Transcription Factor Activity Assay
Eurofins Discoverx cAMP Hunter Liraglutide Bioassay Kit, 2-Plate. (95-0062Y2-00099)
The cAMP Hunter Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses Liraglutide based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress HTR1F U2OS β-Arrestin GPCR Assay, 10-Plate Kit. (93-0699E3CP0L)
The PathHunter HTR1F eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. serotonin) based activation of HTR1F GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged HTR1F and EA-tagged β-Arrestin-2. Activation of HTR1F stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress VIPR2 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0317E2CP0M)
The PathHunter VIPR2 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. VIP) based activation of VIPR2 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged VIPR2 and EA-tagged β-Arrestin-2. Activation of VIPR2 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.